Journal of Nucleic Acids

Research Article

Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta

Table 1

Sequences of DNA substrate s a .
D-1 5 -CGCAGCCGTCCAACCAACTCA- 3
3 -GCGTCGGCAGGTTGGTTGAGTA GCAGCTAGGTTACGGCAGG- 5
D-6 5 -CGCAGCCGTCCAACCAACTCA- 3
3 -GCGTCGGCAGGTTGGTTGAGTG GCAGCTAGGTTACGGCAGG- 5
D-7 5 -CGCAGCCGTCCAACCAACTCA- 3
3 -GCGTCGGCAGGTTGGTTGAGTT GCAGCTAGGTTACGGCAGG- 5
D-8 5 -CGCAGCCGTCCAACCAACTCA- 3
3 -GCGTCGGCAGGTTGGTTGAGTC GCAGCTAGGTTACGGCAGG- 5
F-8 5 -CGCAGCCGTCCAACCAACTCA- 3
3 -GCGTCGGCAGGTTGGTTGAGTCX CAGCTAGGTTACGGCAGG- 5
BE1 5 -ATGAGTTGCAACTCAT- 3
3 -TACTCAACGTTGAGTA- 5
BE2 5 -TTGAGTTGCAACTCAA- 3
3 -AACTCAACGTTGAGTT- 5
BE3 5 -CTGAGTTGCAACTCAG- 3
3 -GACTCAACGTTGAGTC- 5
BE4 5 -GTGAGTTGCAACTCAC- 3
3 -CACTCAACGTTGAGTG- 5
a T he template base highlighted in bold is unique to each strand and X denotes 2-aminopurine.