Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta

Table 2

Rate and equilibrium dissociation constants for the binary complex yPol𝜂⋅DNA at 23°C.

Kinetic Parameter	Truncated yPol𝜂	Full-length yPol𝜂
𝑘on (𝜇M^-1 s^-1)^a	0.62	0.59
𝑘off (s^-1)	0.008 ± 0.001	0.0041 ± 0.0008
𝐾^DNA𝑑 (nM)^b	34 ± 3	16 ± 1
𝐾^DNA𝑑 (nM)^c	13 ± 5	7 ± 4

^aCalculated as 𝑘_off/𝐾^DNA_𝑑. The 𝐾^DNA_𝑑 value was measured from a fluorescence titration assay.
^bEstimated using EMSA.
^cMeasured using a fluorescence titration assay.