Pre-Steady-State Kinetic Analysis of Truncated and Full-Length Saccharomyces cerevisiae DNA Polymerase Eta
Table 6
Comparison of base substitution fidelity for various DNA polymerases.
Polymerase
Polymerase Family
Fidelit
Differenc
Differenc
(kcal/mol
Truncated yPol
Y
3.7 10-4 to 2.9 10-2
3 to 68
5 to 220
1.6
Dpo
Y
1.5 10-4 to 3.2 10-3
1 to 18
240 to 1700
0.95
rPol
X
1.1 10-5 to 5.9 10-4
35 to 342
28 to 708
3.0
PolB1
B
3.5 10-6 to 1.2 10-4
109 to 918
4 to 589
3.7
hPol
A
4.6 10-7 to 2.9 10-4
42 to 900
39 to 12000
3.4
alculated as alculated as defined in equation (8). alculated using equation (7). t 23°C (this work). t 37°C [50]. t 37°C [66]. t 37°C, excluding the fidelity contribution from the exonuclease activity [67]. t 37°C, excluding the fidelity contribution from the exonuclease activity [68].
