Synthetic Pre-miRNA-Based shRNA as Potent RNAi Triggers
Off-target effects induced by synthetic shRNAs. HeLa cells were transfected with the indicated RNAs. After 48 h, the expression of about 20,000 protein-coding genes was analyzed by microarray analysis. The overlap of genes that showed a more than 2-fold decrease in expression as compared with the control siRNA-transfected sample is shown. The number in the overlapping region of the Venn diagram represents shared genes. Note that the LMNA gene is included in the commonly downregulated genes.