Photoswitching of Site-Selective RNA Scission by Sequential Incorporation of Azobenzene and Acridine Residues in a DNA Oligomer
Figure 2
A denaturing 20% PAGE pattern for site-selective scission of 1 activated by 2 or () in the presence of Lu(III) with or without UV irradiation. Lane 1, control in a buffer solution; lane 2, treatment with Lu(III) alone; lane 3, RNase T1 digestion; lane 4, 2 and Lu(III) without UV irradiation; lane 5, 2 and Lu(III) with UV irradiation; lane 6, 4, 5, and Lu(III) without UV irradiation; lane 7, 4, 5, and Lu(III) with UV irradiation; lane 8, 3 and Lu(III) without UV irradiation; lane 9, 3 and Lu(III) with UV irradiation. The 5′-side cleavage of U-19 is indicated by the arrow. Conversions of each scission are presented in the bottom. Reaction conditions; [RNA] = 5 μM, [DNA] = 10 μM, [LuCl3] = 100 μM, [Tris] = 10 mM, [NaCl] = 200 mM, pH 7.5, 37°C, 4 h.
