Photoswitching of Site-Selective RNA Scission by Sequential Incorporation of Azobenzene and Acridine Residues in a DNA Oligomer
Figure 6
A denaturing 20% PAGE pattern for site-selective scission of 1 activated by 6–9. Lane 1, 6 and Lu(III) without UV irradiation; lane 2, 6 and Lu(III) with UV irradiation; lane 3, 7 and Lu(III) without UV irradiation; lane 4, 7 and Lu(III) with UV irradiation; lane 5, 8 and Lu(III) without UV irradiation; lane 6, 8 and Lu(III) with UV irradiation; lane 7, 9 and Lu(III) without UV irradiation; lane 8, 9 and Lu(III) with UV irradiation. The 5′-side cleavage of U-19 is indicated by the arrow. Conversions of each scission are presented in the bottom. Reaction conditions; [RNA] = 5 μM, [DNA] = 10 μM, [LuCl3] = 100 μM, [Tris] = 10 mM, [NaCl] = 200 mM, pH 7.5, 37°C, 7 h.
