Conformational-Dependent and Independent RNA Binding to the Fragile X Mental Retardation Protein

Figure 4

Functional dissection of structural elements in the 5′ end of BC1 RNA. (a) Ten-fold serial treatment of annealed and unannealed 5′BC1-75 RNA with RNase VI starting at 0.1 units/μL (upper panel) or 0.5 units/μL (lower panel). Arrow marks the major conformer. Lane M shows 100 bp molecular weight markers. (b) Secondary structure model of 5′BC1-75 RNA from M-fold.

(a) RNAse V1

(b) 5′BC1-75 RNA