Direct Detection of Thrombin Binding to 8-Bromodeoxyguanosine-Modified Aptamer: Effects of Modification on Affinity and Kinetics
Figure 3
Sensorgrams on injection of 100 nM thrombin with (a) TBA-H-immobilized chip and (b) TBA-Br-immobilized chip. Measurements were conducted in binding buffer at 25°C. Flow rate was 20 μL min−1.