Direct Detection of Thrombin Binding to 8-Bromodeoxyguanosine-Modified Aptamer: Effects of Modification on Affinity and Kinetics
Figure 4
Calibration curves for thrombin on TBA-H-immobilized chip and TBA-Br-immobilized chip. (a) Change in wavelength versus thrombin concentration. (b) Change in wavelength versus log (thrombin concentration). Measurements were conducted in binding buffer at 25°C. Flow rate was 20 μL min−1.
