A Structurally Variable Hinged Tetrahedron Framework from DNA Origami
Figure 4
DNA PAINT measurements on the triangular base of the tetrahedron. (a) Design schematic of the base showing docking sites for complimentary biotin- and ATTO655-conjugated oligonucleotides. The scaffold path is shown in blue, and staples lacking extended docking sequences are in red. Only basal struts 1, 2, and 3 are shown in the full schematic (left), and local detail at docking sites on strut 1 is given (right). The six extended staples containing docking sites for complimentary biotin-nucleotides are shown in purple, with biotin and the affixed nucleotide strand in black. Likewise, the three docking staples for ATTO655 dye are shown in green, while the ATTO655 dye and attached 8-base nucleotide which transiently hybridize to the structures are in light blue. (b) Resolution of triangular structure via DNA PAINT. (Left) Sample frame and (right) superresolution 2D reconstruction of local binding events. In the color scheme on the reconstructions, lighter colors correspond to a higher number of binding events, showing the underlying triangular form matching the schematic arrangement of dyes. For a comparison, the approximate expected distance between docking sites of 60 nm is indicated in the image. Scale bars: 4 μm (left) and 100 nm (right).
