Figure 3: Multiple sequence alignment of the Passiflora sequence with some plant DFR sequences. The identical and similar residues are highlighted on a black and gray background, respectively. NADP-binding domain is underlined. Boxed amino acids have been considered to control the substrate specificity of DFR enzyme [40], and the amino acid residue (indicated by an arrowhead) is especially important for this specificity [41]. The alignment was performed using CLUSTALX and BOXSHADE program.