Triplet Analysis That Identifies Unpaired Regions of Functional RNAs
Figure 2
The RNA library used in this study. (a) The primary sequence of the constant region and the location of the 30 random nucleotides within the 78-nuclotide RNAs that compose the library are indicated. (b) Tat-binding ability of the libraries before selection (0) and after six rounds of the selection (6). Dot blot analysis was carried out with 10 pmol 32P-labeled RNA and 2.5 pmol Tat peptide in 50 μL of the binding buffer. (c) Primary sequence of the 30-nucleotide random region from 17 clones of the library after six rounds of the selection. The frequent triplets after the triplet subtraction, UUG and UGG, are underlined.
