Incorporation of 3-Aminobenzanthrone into 2′-Deoxyoligonucleotides and Its Impact on Duplex Stability
Figure 2
Expanded contour plot of a 300 ms mixing time NOESY spectrum (600 MHz) of the 14-mer dG(N 2)-3ABA duplex dissolved in 100% D2O phosphate buffer, pH 6.9 (left), depicting NOE interactions among the aromatic protons of the duplex. NOE peaks are particularly strong among the aromatic 3ABA protons facilitating their assignment. Especially relevant is the 3ABA(H1-H11) cross-peak which allows identification of the different aromatic rings. Identical region of a DQF-COSY spectrum recorded under the same conditions (right) showing J-coupling interactions that confirms the NOE-based assignments. The arrow points to the position of the H1 diagonal peak, which is missing on the DQF-COSY spectrum due to the absence of coupling interactions. Labels show the specific assignments of the aromatic protons of 3ABA, with the exception of H4 and H6 where it was not possible. The numbering scheme is shown in Figure 1.
