Stabilization of a G-Quadruplex from Unfolding by Replication Protein A Using Potassium and the Porphyrin TMPyP4
Figure 7
Effect of TMPyP4 and RPA on Gq23 in the presence of K+ ions. (a) CD spectra of Gq23 at varying temperatures in the presence of a 1 : 5 molar ratio of Gq23 : TMPyP4. (b) Melt at 292 nm indicates thermal stabilization by TMPyP4 in 100 mM KCl. (c) CD spectra of Gq23 at a 1 : 5 molar ratio of Gq23 : TMPyP4 (black line). Spectra were collected upon addition of increasing molar ratios (0–5) of RPA or (d) RPA-CDE-core. All spectra were recorded in a buffer containing 100 mM KCl.
