Stabilization of a G-Quadruplex from Unfolding by Replication Protein A Using Potassium and the Porphyrin TMPyP4
Figure 9
Effects of telomestatin and RPA on Gq23 with K+ ions. (a) Gq23 : telomestatin 1 : 5 at increasing molar ratios. (b) Melt at 292 nm of Gq23 alone (black) and 1 : 5 Gq23 : telomestatin (red) indicates no thermal stabilization of Gq23 upon addition of telomestatin. (c) Spectra of increasing molar ratios of RPA (0–5) added to above Gq23 : telomestatin complex and (d) spectra of increasing molar ratios of RPA-CDE-core added to the Gq23 : telomestatin complex. All spectra were recorded in 100 mM KCl.
