Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium
Figure 3
Application of the mixture of 3 different siRNAs targeting ESELE, ICAM-1, and VCAM-1 demonstrated the practicability and efficacy of transfection of multiple siRNAs simultaneously. The -axis reflects the respective adhesion receptor expression (in %), whereby untreated cells stimulated with TNF-α (group 2) were set to 100%. -axis: group 1: HLMECs without siRNA transfection and without TNF-α stimulation; group 2: HLMECs without siRNA transfection, but with TNF-α stimulation; group 3: HLMECs transfected with 3 different receptor specific siRNAs and with TNF-α stimulation; group 4: HLMECs transfected with a nonspecific siRNA and with TNF-α stimulation. Each bar represents the mean ± SD of independent experiments.
