Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium
Figure 4
Effects of siRNA and TNF-α treatment on ESELE, ICAM-1, and VCAM-1 mRNA levels. Transcript levels were examined by real-time RT-PCR. Each bar represents the mean ± SD of three independent experiments. Gene expression was normalized in relation to group 1, which was set to the value of 1.00. Group 1: HLMECs without siRNA transfection and without TNF-α stimulation; group 2: HLMECs without siRNA transfection, but with TNF-α stimulation; group 3: HLMECs transfected with a receptor specific siRNA and with TNF-α stimulation; group 4: HLMECs transfected with a nonspecific siRNA and with TNF-α stimulation.
