Small Interfering RNA Efficiently Suppresses Adhesion Molecule Expression on Pulmonary Microvascular Endothelium
Figure 6
Effects of siRNA and TNF-α treatment on binding of neutrophils to confluent layers of HLMECs. The -axis reflects the respective adhesion of neutrophils (in %), whereby untreated cells stimulated with TNF-α (group 2) were set to 100%. -axis: group 1: HLMECs without siRNA transfection and without TNF-α stimulation; groups 2: HLMECs without siRNA transfection, but with TNF-α stimulation; group 3: HLMECs transfected with specific siRNAs targeting ESELE (a), ICAM-1 (b), VCAM-1 (c), or all 3 different siRNAs simultaneously (d) plus TNF-α stimulation; group 4: HLMECs transfected with a nonspecific siRNA and with TNF-α stimulation. Each bar represents the mean ± SD of independent experiments, each performed in triplicate.
