Research Article
Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA
Figure 10
Synthesis of the fully protected linker unit: (i) Na, tert-butyl acrylate, THF, rt, 1 d (34%); (ii) 2-Cyanoethyl-N,N-diisopropropylchlorophosphoramidite, DIPEA, DCM, rt, 1.5 h (quant.); (iii) (a) BMT, MeCN, rt, 1 h; (b) 0.2 M I2 in THF/pyridine/H2O (2 : 1 : 1) (32%).
