Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA
Figure 8
Hybridization of Alexa488-labeled D1L3 and Cy5-labeled A1L3: 3 μM Alexa488-labeled D1L3: 3 μM Cy5-labeled A1L3, 10 mM MgCl2, 50 mM Tris buffer, pH 7.4, total volume 100 μL, 78°C 3 min, slowly cooling to rt. The hybrid was analyzed by 15% native PAA-gel: (left) illuminated at 254 nm, (right) illuminated at 365 nm.
