Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA

Table 3

Sequences of two-stranded or three-stranded amino modified aptazymes. APU: 5-(3-Aminoprop-1-enyl)uridine. AMU: 2′-aminouridine.

Name	Length	Sequence	Mod. X
HPAT2-L12	72 nts	AAA GAG AGA AGU GAA CCA GAG AAA CAG CCU UAG GAU AXG CUU CGG CAG AAG GAC GUC GUA UAU UAC CUG GUA	APU
HPAT2-L14	72 nts	AAA GAG AGA AGU GAA CCA GAG AAA CAG CCU UAG GAX AUG CUU CGG CAG AAG GAC GUC GUA UAU UAC CUG GUA	APU
HPAT2-U12	72 nts	AAA GAG AGA AGU GAA CCA GAG AAA CAG CCU UAG GAU AXG CUU CGG CAG AAG GAC GUC GUA UAU UAC CUG GUA	AMU
HPAT2-U14	72 nts	AAA GAG AGA AGU GAA CCA GAG AAA CAG CCU UAG GAX AUG CUU CGG CAG AAG GAC GUC GUA UAU UAC CUG GUA	AMU
HPAT1-AAM	43 nts	AAA GAG AGA AGU GAA CCA GAG AAA CAG CCU UAG GAX AUG CUG G	AMU
HPAT1-AHI	43 nts	AAA GAG AGA AGU GAA CCA GAG AAA CAG CCU UAG GAX AUG CUG G	APU
HPAT1-BAM	43 nts	AAA GAG AGA AGU GAA CCA GAG AAA CAG CCU UAG GAU AXG CUG G	AMU
HPAT1-BHI	43 nts	AAA GAG AGA AGU GAA CCA GAG AAA CAG CCU UAG GAU AXG CUG G	APU
HPAT2	31 nts	CCA GCA GAA GGA CGU CGU AUA UUA CCU GGU A	—