Synthesis of Specifically Modified Oligonucleotides for Application in Structural and Functional Analysis of RNA

Table 5

Masses of dye-labeled oligonucleotides determined by MALDI-TOF-MS (linear negative-ion mode, found M = [M+H]⁻).

Labeled sequence	Cal. mass	Found mass	Δm	Error (%)
2-L3-A-ATTO 647N	3213.63	3212.44	0.19	0.006
A1L2-ATTO 647N	8783.03	8782.28	0.25	0.003
A2L2-ATTO 647N	8783.03	8776.95	5.08	0.057
A2L2-Cy5	8793.25	8791.53	0.72	0.008