Radiolytic Reduction Characteristics of Artificial Oligodeoxynucleotides Possessing 2-Oxoalkyl Group or Disulfide Bonds
Figure 5
(a,b) Schematic illustration of regulation of DNA polymerase reaction by X-irradiation. The reaction in the presence of caged ODN 7 before X-irradiation (a) or after X-irradiation (b). (c) Sequences of oligodeoxynucleotides used for DNA polymerase reaction. (d) Representative gel electrophoresis of DNA elongation by the Klenow fragment of DNA polymerase I. ODN 7 (100 μM) was X-irradiated (1200 Gy) under hypoxic conditions, and then ODN 6 (5 μM), fluorescein-labeled ODN 5 (5 μM), and enzyme (0.2 unit) were added. After the incubation for 15 min at 32°C, the elongation was monitored by gel electrophoresis. Lane 1: marker (20 mer), Lane 2: the ODN 7 was added before X-irradiation. Lane 3: the ODN 7 was added after X-irradiation (1200 Gy).
