The Effects of Molecular Crowding on the Structure and Stability of G-Quadruplexes with an Abasic Site
Table 2
Thermodynamic parameters of AP series for the formation of G-quadruplexa.
PEG200 (wt%)
b (°C)
(kcal mol−1)
(kcal mol−1)
(kcal mol−1)
c (kcal mol−1)
d (kcal mol−1)
AP-0
0
58.1
−47.9 ± 0.3
−44.8 ± 0.3
−3.1 ± 0.4
−2.3 ± 1.0
—
40
66.9
−62.1 ± 1.0
−56.6 ± 0.8
−5.5 ± 1.0
—
—
AP-1
0
52.2
−41.3 ± 0.4
−39.4 ± 0.5
−1.9 ± 0.6
−1.6 ± 1.0
1.2 ± 0.7
40
61.9
−46.5 ± 0.6
−43.0 ± 0.6
−3.5 ± 0.8
—
1.9 ± 1.2
AP-2
0
44.2
−40.8 ± 0.5
−39.9 ± 0.5
−0.9 ± 0.3
−1.5 ± 1.0
2.2 ± 0.5
40
53.2
−48.2 ± 0.8
−45.8 ± 0.7
−2.4 ± 0.8
—
3.0 ± 1.3
AP-3
0
50.0
−42.1 ± 0.6
−40.4 ± 0.6
−1.7 ± 0.7
−1.2 ± 1.5
1.4 ± 0.8
40
57.2
−47.9 ± 1.0
−45.0 ± 0.9
−2.9 ± 1.4
—
2.5 ± 1.7
AP-4
0
39.1
−38.6 ± 0.3
−38.3 ± 0.3
−0.3 ± 0.3
−1.1 ± 0.3
2.8 ± 0.5
40
46.2
−47.9 ± 0.4
−46.3 ± 0.3
−1.4 ± 0.3
—
4.0 ± 1.0
AP-5
0
30.9
−32.6 ± 0.3
−33.3 ± 0.3
0.7 ± 0.3
−1.7 ± 0.6
3.8 ± 0.5
40
43.8
−46.1 ± 0.4
−45.1 ± 0.4
−1.0 ± 0.5
—
4.4 ± 1.1
AP-6
0
47.0
−40.7 ± 0.4
−39.4 ± 0.4
−1.3 ± 0.6
−1.5 ± 0.9
1.8 ± 0.7
40
56.1
−48.1 ± 0.6
−45.3 ± 0.6
−2.8 ± 0.7
—
2.6 ± 1.2
AP-7
0
43.5
−45.1 ± 0.4
−44.2 ± 0.4
−0.9 ± 0.5
−1.3 ± 0.9
2.2 ± 0.6
40
51.3
−48.4 ± 0.4
−46.1 ± 0.4
−2.2 ± 0.5
—
3.2 ± 1.1
AP-8
0
33.5
−31.3 ± 0.3
−31.7 ± 0.3
0.4 ± 0.3
−1.8 ± 0.6
3.5 ± 0.5
40
46.7
−46.4 ± 0.3
−45.0 ± 0.3
−1.4 ± 0.3
—
4.0 ± 1.0
AP-9
0
41.1
−35.2 ± 0.2
−33.3 ± 0.3
−0.5 ± 0.3
−1.4 ± 0.4
2.6 ± 0.5
40
49.5
−47.5 ± 0.4
−45.6 ± 0.3
−1.9 ± 0.3
—
3.5 ± 1.0
AP-10
0
38.8
−37.5 ± 0.4
−37.3 ± 0.4
−0.2 ± 0.5
−1.3 ± 0.6
2.9 ± 0.6
40
48.0
−44.8 ± 0.3
−43.3 ± 0.3
−1.5 ± 0.4
—
3.9 ± 1.1
AP-11
0
36.4
−33.3 ± 0.7
−33.5 ± 0.7
0.2 ± 0.7
−1.9 ± 1.1
3.3 ± 0.9
40
48.1
−42.8 ± 0.7
−41.1 ± 0.6
−1.7 ± 0.7
—
3.7 ± 1.2
aThe error values were calculate as shown in [44].
b is the melting temperature at 20 μM total strand concentration.
c.
d ( indicates the numbers from 1 to 11).
