A Potential of microRNAs for High-Content Screening

Table 2

miRNA-based functional screens.

Model system	Number of miRNAs screened	Type of regulation	Phenotype measured	Assay	Year	Reference
HeLa and A549	95	Loss-of-function by miRNA inhibitors	Cell proliferation and apoptosis	Cell counting and caspase-3/7 activity assay	2005	[100]
Primary hTERT-immortalized BJ-EHT fibroblasts	~450	Gain-of-function by stable miRNA expression	Sustained proliferation	miR-Array	2006	[40]
HeLa	~450	Gain-of-function by stable miRNA expression	p27^Kip1 regulation	GFP reporter assay and miR-Array	2007	[45]
MDA-MB-453	187	Gain-of-function by miRNA precursors	TRAIL-induced caspase-3 activation	Caspase-3/7 activity assay	2007	[104]
Drosophila clone 8 cells	77/78 miRNA loci	Gain-of-function by plasmid-based miRNA expression	Wg signaling pathway regulation	Luciferase reporter assay	2007	[110]
Neuroblastoma cell lines	8	Gain- and loss-of-function by miRNA mimics and inhibitors	Proliferation	Change in electrical impedance	2008	[111]
MCF7	~450	Gain-of-function by stable miRNA expression	Cell migration	Trans-well cell migration assay	2008	[101]
HeLa	91	Gain-of-function by miRNA mimics	p53 gene activity	Luciferase reporter assay	2009	[38]
HeLa	~450	Gain-of-function by stable miRNA expression	Per gene family regulation	GFP reporter assay and miR-Array	2009	[102]
Primary ovarian granulosa cells	80	Gain-of-function by miRNA precursors	Progesterone, testosterone and estradiol release	Enzyme immunoassay (EIA)	2009	[112]
HEK 293	266	Gain-of-function by miRNA mimics	p21Cip/Waf1 regulation	Luciferase reporter assay	2010	[46]
HCT-16	810	Gain-of-function by miRNA mimics	Cell viability in the presence of Bcl-2 family inhibitor ABT-263	CellTiter-Glo Luminescent Cell Viability Assay	2010	[99]
HEK 293T	107	Gain-of-function by miRNA mimics	p53 gene regulation	Luciferase reporter assay	2010	[39]
HCT116 p53^+/+, H460 and MCF7	5	Gain-of-function by miRNA precursors	p53 gene regulation	Western blot	2010	[10]
Huh-7	327	Gain-of-function by miRNA precursors	Lipid droplet formation and growth	Immunocytochemistry and fluorescence microscopy	2010	[105]
Primary ovarian granulosa cells	80	Gain-of-function by miRNA precursors	Proliferation and apoptosis	Immunocytochemistry and fluorescence microscopy	2010	[113]
DLD-1	319	Gain-of-function by miRNA precursors	Cell viability	CellTiter-Glo Luminescent Cell Viability Assay	2010	[11]
HMEC	328	Gain-of-function by miRNA precursors	Proliferation	Fluorescence microscopy	2010	[114]
MIA PaCa-2	445	Gain-of-function by stable miRNA expression	Proliferation	Custom-made microarray	2010	[115]
HeLa and HeLa P4	8	Gain- and loss-of-function by miRNA precursors and inhibitors	Cell proliferation and trafficking	Fluorescence microscopy	2011	[116]