Journal of Nucleic Acids / 2011 / Article / Tab 3 / Review Article
A Potential of microRNAs for High-Content Screening Table 3 Validation assays in miRNA-based screens.
Effector miRNA Target genes Validation assay Year Reference Expressiona Reporterb qRT-PCR ISc siRNAd miR-372 and -373 LATS2 + + + + 2006 [40 ] miR-221 and -222 p27Kip1 + + 2007 [45 ] miR-315 Axin and Notum + 2007 [110 ] miR-34a Bcl2 and MYCN + 2008 [111 ] miR-373 and -520c CD44 + + 2008 [101 ] miR-29a, b, c p85α and CDC42 + + 2009 [38 ] miR-192 and -194 Per1, 2 and 3 + 2009 [102 ] 28 miRNAs p21Cip/Waf1 + + 2010 [46 ] 10 miRNAs MCL1 + 2010 [99 ] miR-1285 p53 + + + 2010 [39 ] miR-504 p53 + + 2010 [10 ] 11 miRNAs Multiple genes + 2010 [105 ] miR-491 Bcl-xL + + + 2010 [11 ] 28 miRNAs p21Cip/Waf1 + + 2010 [114 ]
a mRNA expression profiling.
b Luciferase and/or GFP reporter assays.
c Immunostaining.
d Recapitulation of miRNA-mediated phenotype by selected siRNAs.