Identification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA

Figure 4

FP ssDNA-binding assays for RPA, RPA-AB, and RPA-CDE-core at 100 mM NaCl. Representative binding isotherms are shown. (a)–(d) RPA, (e)–(h) RPA-AB, and (i)–(l) RPA-CDE-core. The ssDNA sequences (Gq23, Comp, polyA, or polyG) are displayed at the top of each column, and the protein construct is listed at the beginning of a row. For each FP reaction, 0.060 μM of oligo was titrated with ~0–50 μM of protein, and a binding isotherm was generated. 𝐾_𝑑 values for each experiment are listed on the top left of each binding isotherm.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)