Identification of the DNA-Binding Domains of Human Replication Protein A That Recognize G-Quadruplex DNA
Figure 6
CD experiments for Gq23 oligonucleotide melting and protein titrations with RPA-CDE, RPA-DE, and RPA-C. (a) Melting of Gq23, (b) Titration of Gq23 with RPA-CDE at varying protein:ssDNA ratios. Peak at ~292 nm indicates the presence of an antiparallel G-quadruplex. Parts (a) and (b) were collected in Buffer A with 100 mM NaCl. (c) Melting of Gq23 in 10 mM NaCl, (d) Titration of Gq23 with RPA-DE, and (e) Titration of Gq23 with RPA-C. Parts (c–e) were collected in Buffer A with 10 mM NaCl. Note, spectra contain both antiparallel and parallel (254 nm) G-quadruplex peaks [10].
