Research Article
Coupling Strategies for the Synthesis of Peptide-Oligonucleotide Conjugates for Patterned Synthetic Biomineralization
Table 1
Reaction yields for POC formation in the listed conditions.
| Reaction | Coupling reagent | Solvent | Supporting media | Temperature | Reaction time | Yield (%) |
| 1 | HBTU | DMF | Sepharose | room temperature | 16 h | 0 | 2 | PyBOP | DMF | Sepharose | room temperature | 16 h | 4 | 3 | DMTMM | Water | Sepharose | room temperature | 16 h | 15 | 4 | EDC | Water | Sepharose | room temperature | 16 h | 24 | 5 | DMTMM | Water | Sepharose | 50°C | 16 h | 15 | 6 | DMTMM | Water | Sepharose | room temperature | 2 h | 15 | 7 | PyBOP | DMF | Sepharose | room temperature | 2 h | 4 | 8 | DMTMM | Water | Solution | room temperature | 16 h | 50 | 9 | EDC | Water | Solution | room temperature | 16 h | 95 | 10 | DMTMM | Water | Solution | 50°C | 16 h | 55 |
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