Journal of Nucleic Acids / 2012 / Article / Tab 2

Review Article

Dioxaphosphorinane-Constrained Nucleic Acid Dinucleotides as Tools for Structural Tuning of Nucleic Acids

Table 2

Sequences and melting temperatures of CNAgm containing duplexes.

EntrySequencea (°C) (°C)

15′-GCGTTTTTTGCT-3′  
3′-CGCAAAAAACGA-5′
49.0
25′-GCGTTTxT TTGCT-3′  
3′-CGCAAAAAACGA-5′
55.0+6.0
35′-GCGTTTxTTxT GCT-3′  
3′-CGCAAAAAACGA-5′
59.0+10.0
45′-GCGTxTTxTTxT GCT-3′  
3′-CGCAAAAAACGA-5′
64.0+15.0
55′-GCAAAAACTTGC-3′  
3′-CGTTTTTGAACG-5′
48.0
65′-GCAAAAACTxT GC-3′  
3′-CGTTTTTGAACG-5′
53.2+5.2
75′-GCAAAAACTTGC-3′  
3′-CGTTTTxT GAACG-5′
52.2+4.2
85′-GCAAAAACTTGC-3′  
3′-CGTTTxT TGAACG-5′
54.3+6.3
95′-GCAAAAACTTGC-3′  
3′-CGTTxT TTGAACG-5′
53.4+5.4
105′-GCAAAAACTTGC-3′  
3′-CGTxT TTTGAACG-5′
52.4+4.4
115′-GCAAAAACTxT GC-3′  
3′-CGTTTTxT GAACG-5′
55.9+7.9
125′-GCAAAAACTxT GC-3′  
3′-CGTTTxT TGAACG-5′
56.4+8.4
135′-GCAAAAACTxT GC-3′  
3′-CGTTxT TTGAACG-5′
57.6+9.6
145′-GCAAAAACTxT GC-3′  
3′-CGTxT TTTGAACG-5′
57.8+9.8

aTxT : ( ) -D-CNA TT (CNAgm) within the strand. bUV melting experiments were carried out in sodium phosphate buffer (10 mM, pH 7.0) containing NaCl (100 mM) and EDTA (1 mM).

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