Journal of Nucleic Acids / 2012 / Article / Tab 3

Review Article

Dioxaphosphorinane-Constrained Nucleic Acid Dinucleotides as Tools for Structural Tuning of Nucleic Acids

Table 3

Comparison of melting temperatures of CNAgm or CNAgp containing duplexes.

Entry Sequencea   °C (   °C)

1dA14/dT4TxT T424.230.1 (+5.9)10.5 (−13.7)
2dA14/dT6TxT T635.038.1 (+3.1)24.0 (−11.0)
35′-GCGCTxT GCCG-3′  
3′-CGCGAACGGC-5′
53.058.0 (+5.0)44.0 (−9.0)
45′-ACATTxT GAAATGCAAATG-3′  
3′-TGTAAACTTTACGTTTAC-5′
49.052.0 (+3.0)44.1 (−4.9)
55′-ACATTTGAAATGCAAATG-3′  
3′-TGTAAACTTTACGTxT TAC-5′
49.054.9 (+5.9)43.3 (−5.7)
65′-ACATTTGAAATGCAAATG-3′  
3′-TGTAAACTxT TACGTTTAC-5′
49.054.5 (+5.5)49.0 (0.0)
75′-CTCATGAATATGCAAATC-3′  
3′-GAGTACTTATACGTxT TAG-5′
52.455.0 (+2.6)47.2 (−5.2)
85′-CTCATGAATATGCAAATC-3′  
3′-GAGTACTxT ATACGTTTAG-5′
52.456.5 (+4.1)47.6 (−4.8)
95′-TGCTCAGTAAATAATGCA-3′  
3′-ACGAGTCATTTATxT ACGT-5′
55.659.0 (+3.4)49.3 (−6.3)
105′-TGCTCAGTAAATAATGCA-3′  
3′-ACGAGTCATxT TATTACGT-5′
55.658.8 (+3.2)49.6 (−6.0)
115′-ATCTCATTTGAAATGCAAATGGAA-3′  
3′-TAGAGTAAACTTTACGTxT TACCTT-5′
57.959.3 (+1.4)53.5 (−4.4)
125′-TGTCTCATGAATATGCAAATCACA-3′  
3′-ACAGAGTACTTATACGTxT TAGTGT-5′
59.961.3 (+1.4)55.7 (−4.2)
135′-CGCGAATxT CGCG-3′  
3′-GCGCTxT AAGCGC-5′
62.0c58.0 (−4.0)c27.0 (−35.0)
82.0 (+20.0)

aTxT : modified TT within the strand. bUV melting experiments were carried out in sodium phosphate buffer (10 mM, pH 7.0) containing NaCl (100 mM) and EDTA (1 mM). cOnly one transition observed.

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