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Journal of Nucleic Acids
Volume 2012, Article ID 247280, 7 pages
http://dx.doi.org/10.1155/2012/247280
Research Article

Nucleic-Acid-Binding Chromophores as Efficient Indicators of Aptamer-Target Interactions

1Department of Chemistry, Missouri State University, 901 South National Avenue, Springfield, MO 65897, USA
2Department of Chemistry, Indian Institute of Technology Gandhinagar, VGEC Complex, Chandkheda, Ahmedabad 382424, India

Received 20 June 2012; Revised 1 September 2012; Accepted 3 September 2012

Academic Editor: Ramón Eritja

Copyright © 2012 Kwabena Sarpong and Bhaskar Datta. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The binding affinity and specificity of nucleic acid aptamers have made them valuable candidates for use as sensors in diagnostic applications. In particular, chromophore-functionalized aptamers offer a relatively simple format for detection and quantification of target molecules. We describe the use of nucleic-acid-staining reagents as an effective tool for detecting and signaling aptamer-target interactions. Aptamers varying in size and structure and targeting a range of molecules have been used in conjunction with commercially available chromophores to indicate and quantify the presence of cognate targets with high sensitivity and selectivity. Our assay precludes the covalent modification of nucleic acids and relies on the differential fluorescence signal of chromophores when complexed with aptamers with or without their cognate target. We also evaluate factors that are critical for the stability of the complex between the aptamer and chromophore in presence or absence of target molecules. Our results indicate the possibility of controlling those factors to enhance the sensitivity of target detection by the aptamers used in such assays.