Nucleic-Acid-Binding Chromophores as Efficient Indicators of Aptamer-Target Interactions
Figure 6
Fluorescence signaling by chromophores in presence of aptamers exposed to variable amounts of cognate target. Chromophores used for (a) ATP, (b) theophylline, and (c) thrombin were, respectively, Hoechst 33258, TO, and SYBR Green I. The % decrease in fluorescence is calculated from the difference in fluorescence intensities of 25 nM chromophores in presence of 10 nM aptamer and variable amounts of cognate target.
