Table 1: Sequences of aptamers used and their affinities for corresponding target.

TargetSequenceAffinity ( )Expected secondary structure

ATP [17]5′-ATAGCGGAGGAAGGATACCTGGGGGAGTATAT-3′6 μMQuadruplex, stem-loop
Thrombin [18]5′-GGTTGGTGTGGTTGG-3′10 nMQuadruplex
Theophylline [21]5′-GGUGAUACCAGCAUCGUCUUGAUGCCCUUGGCAGCACC-3′220 nMHairpin, stem-loop