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Journal of Nucleic Acids
Volume 2012 (2012), Article ID 254630, 4 pages
http://dx.doi.org/10.1155/2012/254630
Research Article

Straightforward Procedure for Laboratory Production of DNA Ladder

Faculty of Biology, Hanoi University of Science, Vietnam National University, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, Vietnam

Received 17 September 2011; Revised 27 October 2011; Accepted 28 October 2011

Academic Editor: Ramón Eritja

Copyright © 2012 Vo Thi Thuong Lan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

DNA ladder is commonly used to determine the size of DNA fragments by electrophoresis in routine molecular biology laboratories. In this study, we report a new procedure to prepare a DNA ladder that consists of 10 fragments from 100 to 1000 bp. This protocol is a combination of routinely employed methods: cloning, PCR, and partial digestion with restriction enzymes. DNA fragments of 100 bp with unique restriction site at both ends were self-ligated to create a tandem repeat. Once being cloned, the tandem repeat was rapidly amplified by PCR and partially digested by restriction enzymes to produce a ladder containing multimers of the repeated DNA fragments. Our procedure for production of DNA ladder could be simple, time saving, and inexpensive in comparison with current ones widely used in most laboratories.