UvrD Participation in Nucleotide Excision Repair Is Required for the Recovery of DNA Synthesis following UV-Induced Damage in Escherichia coli
UvrD is required for the recovery of replication following UV irradiation, but not for replication in the absence of damage. Cells grown in the presence of [14C]-thymine were pulse-labeled for 2 min with [3H]-thymidine at the times indicated following either UV irradiation with 27 J/m2 (open symbols) or mock irradiation (closed symbols). Total DNA accumulation (14C incorporation, circles) and rate of synthesis (3H incorporation/ 2 min, squares) are plotted. Graphs represent the average of at least three independent experiments. Error bars represent the standard error of the mean. The level of [3H] and [14C] in preirradiated DNA ranged between 30,000–50,000 cpm and 3000–6000 cpm for all experiments.
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