Journal of Nucleic Acids / 2012 / Article / Fig 2

Research Article

UvrD Participation in Nucleotide Excision Repair Is Required for the Recovery of DNA Synthesis following UV-Induced Damage in Escherichia coli

Figure 2

UvrD is required for the recovery of replication following UV irradiation, but not for replication in the absence of damage. Cells grown in the presence of [14C]-thymine were pulse-labeled for 2 min with [3H]-thymidine at the times indicated following either UV irradiation with 27 J/m2 (open symbols) or mock irradiation (closed symbols). Total DNA accumulation (14C incorporation, circles) and rate of synthesis (3H incorporation/ 2 min, squares) are plotted. Graphs represent the average of at least three independent experiments. Error bars represent the standard error of the mean. The level of [3H] and [14C] in preirradiated DNA ranged between 30,000–50,000 cpm and 3000–6000 cpm for all experiments.

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