Expression Profiling of a Heterogeneous Population of ncRNAs Employing a Mixed DNA/LNA Microarray

Figure 1

Self-self hybridization at 56°C of 5 μg (per dye) of labeled total mouse brain RNA. Diagrams of average intensity values show filtered results in logarithmic scale. The 𝑦 axis represents values of AlexaFluor3 dye measurement at 532 nm, and the 𝑥 axis represents values of AlexaFluor5 dye measurement at 635 nm. Red spots represent all signals from LNA and DNA probes spotted on the microarray slide. (a, c) Detection of MiR-9 (yellow spots, black arrow) and miR-9* (yellow spots, white arrow) with LNA probes. (b, d) Detection of snoRNAs (yellow spots) with antisense DNA probes (including mismatch probes in (d)). Sense DNA probes signals were below detection levels and thus filtered out. (a, b) RNA labeling using the protocol described by manufacturer. (c, d) Modified RNA labeling (see Section 2).