MsDpo4—a DinB Homolog from Mycobacterium smegmatis—Is an Error-Prone DNA Polymerase That Can Promote G:T and T:G Mismatches
Figure 3
Fidelity profile of MsDpo4. (a) The results for primer extension assay wherein MsDpo4 was incubated with DNA duplexes (T1P1, T2P1, T3P1, and T4P1) that presented different template nucleotides in the active site (G, T, C, and A) are displayed. This mixture of MsDpo4 and DNA either included all deoxynucleotide triphosphates (denoted as N) or the four different dNTPs separately (G, T, C, and A). (b) Bar diagram exhibiting the incorporation profile of MsDpo4. Although the Watson-Crick base pairing is the preferred mode of nucleotide selection, MsDpo4 does exhibit significant ability to promote G:T and T:G mismatches.
