Review Article

Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems

Figure 5

Phage display screening with next-generation sequencing. (a) Biopanning with one or two cycle(s). (b) Randomized region of phage DNA is amplified with Polymerase Chain Reaction (PCR). The products are subjected to gel electrophoresis followed by further DNA purification. (c) Purified DNA is analyzed by a next-generation sequencer.
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