Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems

Table 1

Features of various systems of M13 and T7 phages.

	System	Size limit	Numbers of peptides per virion	Presentation region
M13	3	Unknown	5	N-terminus to gp3
	33	No limit	<1
	3+3
	8	Short	>2,700	N-terminus to gp8
	88	Unknown	<300
	8+8	No limit	100–1000
	8+8	Unknown		C-terminus to gp8
	6+6	No limit	<1	N-terminus to gp6
	6+6			C-terminus to gp6
	9+9			N-terminus to gp9
T7	T7Select1-1	1200aa	<1	C-terminus to gp10B
	T7Select1-2	900aa
	T7Select10-3	1200aa	5–15
	T7Select415-1	50aa	415