Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems
Table 3
Codon usage in E. coli K-12 strain.
Amino acid
Codon
Codon frequency (%)
Phe
UUU
1.97
UUC
1.50
Leu
UUA
1.52
UUG
1.19
CUU
1.19
CUC
1.05
CUA
0.53
CUG
4.69
Ile
AUU
3.05
AUC
1.82
AUA
0.37
Met
AUG
2.48
Val
GUU
1.68
GUC
1.17
GUA
1.15
GUG
2.64
Ser
UCU
0.57
UCC
0.55
UCA
0.78
UCG
0.80
AGU
0.72
AGC
1.66
Pro
CCU
0.84
CCC
0.64
CCA
0.66
CCG
2.67
Thr
ACU
0.80
ACC
2.28
ACA
0.64
ACG
1.15
Ala
GCU
1.07
GCC
3.16
GCA
2.11
GCG
3.85
Tyr
UAU
1.68
UAC
1.46
His
CAU
1.58
CAC
1.31
Gln
CAA
1.21
CAG
2.77
Asn
AAU
2.19
AAC
2.44
Lys
AAA
3.32
AAG
1.21
Asp
GAU
3.79
GAC
2.05
Glu
GAA
4.37
GAG
1.84
Cys
UGU
0.59
UGC
0.80
Stop
UAA
0.18
UAG
0.00
UGA
0.10
Trp
UGG
1.07
Arg
CGU
2.11
CGC
2.60
CGA
0.43
CGG
0.41
AGA
0.14
AGG
0.16
Gly
GGU
2.13
GGC
3.34
GGA
0.92
GGG
0.86
Codon frequency (%) is defined as the percent frequency of each codon which matches in whole open-reading frame of the E. coli K-12 genome. Minor codons (bold letters; below 0.5%) could be avoided for insert DNA construction. This table was cited from codon usage database (http://www.kazusa.or.jp/codon/) with some modifications.
