Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems

Table 4

Comparison of the M13 and T7 phage in library construction and affinity selection.

	M13 phage	T7 Phage
Cost	Routinely cheap, requires	Routinely expensive, no additional
	electroporator and cuvettes	instruments required
Site of library peptide	N-terminus of gp3	C-terminus of gp10B
Library size (per 𝜇g DNA)	~10^9	~10^8 [2]
Peptide sequence bias	Highly biased	Less biased
Time required for phage tittering/amplification	Long	Short