A Two-Piece Derivative of a Group I Intron RNA as a Platform for Designing Self-Assembling RNA Templates to Promote Peptide Ligation
Figure 3
Gel mobility shift assay of the two-piece Tetrahymena RNA consisting of three RNA molecules. (a) The Tet L-30 RNA and a bimolecular derivative (P5 + ΔP5) were used as positive controls. Electrophoresis was carried out with 10 mM Mg2+ ions. To visualize RNA molecules on the gel, the 3′ ends of FL-labeled RNAs were labeled with BODIPY. (b) Influence of PRM1 and PRM2 on the P5 : Ez complex formation. Electrophoresis was carried out with 10 mM Mg2+ ions. To visualize RNA molecules on the gel, the 3′ ends of FL-labeled RNAs were labeled with BODIPY.
