Chemical Approaches for Structure and Function of RNA in Postgenomic Era
Figure 22
Nucleotide composition of U1 5′ oligonucleotide [16]. The peak from the GTP region was digested with snake venom phosphodiesterase and separated on Whatman 3MM paper by electrophoresis at pH 3.5 (5% acetic acid adjusted pH to 3.5 with ammonium hydroxide) and chromatography in the second dimension with a solvent system consisting of isopropyl alcohol, HCl, and H2O in the ratio of 680 : 176 : 144 by volume. Autoradiography was performed using X-ray film.
