Chemical Approaches for Structure and Function of RNA in Postgenomic Era

Table 10

The [³²P]cpm radioactivity distribution in nucleotides and cap core produced by RNase P1 digestion of U1 5′ oligonucleotide [16]. The [³²P]-labeled U1 5′ oligonucleotide obtained by digestion of U1 RNA with RNase T2, RNase U2, and alkaline phosphatase was treated with P₁ nuclease which cleaves all phosphodiester bonds but not pyrophosphate bonds. The products were separated on a DEAE column (Figure 23). The radioactivity in peak a (mononucleotides pUm, pA) and peak b (cap core m_32,2,7GpppAm) were determined by Packard liquid scintillation spectrometer.

	Peak a (pUm, pA)	Peak b (m32,2,7GpppAm)
U1 5′ cap	65,280	87,600