Journal of Nucleic Acids / 2012 / Article / Tab 1 / Research Article
In Vitro Selection of Fab Fragments by mRNA Display and Gene-Linking Emulsion PCRTable 1 Oligonucleotide sequences.
Name Sequence (5′ to 3′) Universal-OL1 GAAATTAATACGACTCACTATAGGGAAACCACTACGGTCTCCC TCTAGAAATAATTTTGTTTAACTTTAAGAAG GAGATATACCA Myc-R AAGGTCTTCTTCACTAATCAGTTTCTGCTC Universal-OL2 GAAATTAATACGACTCACTATAGGGAGACCGTAGTGGTTTCCC TCTAGAAATAATTTTGTTTAACTTTAAGAAG GAGATATACCA FLAG-R CTTGTCGTCATCGTCCTTGTAGTC Myc25A-R TTTTTTTTTTTTTTTTTTTTTTTTTAAGGTCTTCTTCACTAATCAGTTTCTGCTC FLAG25A-R TTTTTTTTTTTTTTTTTTTTTTTTTCTTGTCGTCATCGTCCTTGTAGTC PolyT oligo TTTTTTTTTTTTTTTTTTTTTTTTT UnivOL1-F GAAATTAATACGACTCACTATAGGGAAACCACTACGGTC UnivOL2-F GAAATTAATACGACTCACTATAGGGAGACCGTAGTGGTT Fluo-F CAGGATCGAGTGGGTCAAAC Fluo-R GGTGCTGGAGCTTTTGTCTG p53-F GGCAGAGCTTGTAAGGTCAG p53-R CAATCCATCCAATCCACTCC
The bold type resembles the sequences of the overlapping region for overlap-extension PCR. Poly T oligo is labeled with a photo-cleavable biotin at the 5′ end.