Journal of Nucleic Acids / 2012 / Article / Fig 1

Research Article

Structural and Functional Characterization of RecG Helicase under Dilute and Molecular Crowding Conditions

Figure 1

CD spectra of 5 μM RecG. Measurements were carried out in 30 mM MES buffer (pH 7.0) containing 100 mM Na+ and 0.5 mM Na2EDTA (a) at 0 wt% PEG 200 without ATP at 4°C (black) and 37°C (green), or with 1 mM ATP at 4°C (red) and 37°C (blue), and (b) at 40 wt% PEG 200 without ATP at 4°C (black) and 37°C (green), or with 1 mM ATP at 4°C (red) and 37°C (blue), respectively.
392039.fig.001a
(a)
392039.fig.001b
(b)

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