Structural and Functional Characterization of RecG Helicase under Dilute and Molecular Crowding Conditions
Figure 1
CD spectra of 5 μM RecG. Measurements were carried out in 30 mM MES buffer (pH 7.0) containing 100 mM Na+ and 0.5 mM Na2EDTA (a) at 0 wt% PEG 200 without ATP at 4°C (black) and 37°C (green), or with 1 mM ATP at 4°C (red) and 37°C (blue), and (b) at 40 wt% PEG 200 without ATP at 4°C (black) and 37°C (green), or with 1 mM ATP at 4°C (red) and 37°C (blue), respectively.
