A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in an In Vitro Compartmentalized Cell-Free Translation System
Figure 1
(a) Schematic sequence of the tRNA-fused template with two T7 promoters. The triple N in red represents the anticodon- or anticodon-loop-randomized region. (b) Agarose gel electrophoretic assay of the template DNAs recovered from tRNACUA-fused template (lane 1), nonfused template lacking the tRNA domain (lane 2), a 1 : 1 mixture thereof (lanes 4, 5), or -fused template (lane 3), after coupled transcription/translation under normal (noncompartmentalized) (lanes 1–4) or compartmentalized (lane 5) conditions.
