Inhibition of HIV Replication by Cyclic and Hairpin PNAs Targeting the HIV-1 TAR RNA Loop
Figure 6
Dose-effect curves and median-effect plots for antiviral activity of TPP-conjugates. CEM cells were infected with VSV-G pseudotyped S1 strain of HIV-1 and grown in the presence of increasing concentrations of test conjugates. After 48 h, cells were harvested and assayed for luciferase expression using Luciferase assay kit (Promega). The effect of conjugate concentrations on the luciferase expression was analyzed using CalcuSyn software (Biosoft). The IC50 values, determined from the ratio of log of luciferase expression in treated cells (fa) and untreated cells (fu) as a function of the log concentration of conjugates was 1.0 > M; the linear correlation coefficient, r, ranged from 0.943 to 0.985. (a) Dose-effect curves for antiviral activity of TPP conjugates. (b) Median-effect plot.
