Functional Annotation of Small Noncoding RNAs Target Genes Provides Evidence for a Deregulated Ubiquitin-Proteasome Pathway in Spinocerebellar Ataxia Type 1
miRNAs and ncRNAs reduce the expression of HECTD1 in HEK29 3T cells. (a) Schematic diagram of HECTD1 3′UTR depicting the location of relevant miRNA response elements and miR-E1108 and miR-E1016 binding sites. (b) Luciferase assay using HECTD1 3′UTR reporter and miRNA mimics. (c) RT-PCR analysis of HECTD1 and GAPDH expression after transfection with miR-E1108, miR-E1016, and siHECTD1. Relative fold changes in HECTD1 protein levels after transfection with miRNA mimics are shown in lower panel. (d) RT-PCR analysis of HECTD1 and GAPDH after transfection with miR-16, miR-9, and miR-130. Relative fold changes in HECTD1 protein levels after transfection with miRNA mimics are shown in lower panel.
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